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recombinant human epiregulin protein repr  (R&D Systems)


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    R&D Systems recombinant human epiregulin protein repr
    Fig. 1. EGFR signaling suppresses osteoblast differentiation through Ras. A) Expression of EGF family members mRNA in osteoblasts. MC3T3-E1 cells were induced to differentiate into osteoblasts, and the mRNA expression of EGF family members was monitored by RT-PCR at the indicated time points. GAPDH was used as an internal control. Hb-egf, heparin-biding EGF-like growth factor; Tgfα, transforming growth factor-α; Areg, amphiregulin; Epr, <t>epiregulin;</t> Btc, betacellulin. P, positive control. mRNA from mouse heart, liver, and lung was used as a positive control. B) Effect of rHB-EGF on osteoblastogenesis. MC3T3-E1 cells were seeded on 24-well plates, and osteoblast differentiation was induced. Alkaline phosphatase (ALP) activity and mineralizing activity were measured by ALP staining (upper panel) and Alizarin red staining (lower panel), respectively, at the indicated time points. C) Expression of osteoblastic marker genes in MC3T3-E1 cells. Cells were cultured in osteogenic media in the presence of rHB-EGF, and the mRNA expression of the indicated genes was monitored by RT-PCR. ColI, Collagen type I; Alp, alkaline phosphatase; Opn, osteopontin; Ocn, osteocalcin. GAPDH was used as an internal control. D) Activation of ERK in osteoblast differentiation. The phosphorylated form of ERK was visualized by Western blotting. MC3T3-E1 cells were stimulated with rHB-EGF for 5 min in the presence of the indicated inhibitors. A.A., ascorbic acid; HB, rHB-EGF; αHB, anti-human HB-EGF neutralizing antibody; PD, PD98059, MEK inhibitor; AG, AG1478, EGFR inhibitor. E) MC3T3-E1 cells were cultured in osteogenic media stimulated with rHB-EGF and indicated inhibitors as shown in D) for 3 days and ALP staining was carried out. F) Involvement of Ras in osteoblast differentiation. A dominant-negative form of Ras was expressed in MC3T3-E1 cells, and ALP staining was performed three days after osteogenic stimulation in the presence or absence of rHB-EGF. Mock, empty vector.
    Recombinant Human Epiregulin Protein Repr, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+epiregulin+protein+repr/Recombinant+Human+Epiregulin+Protein/pm20448405-37-5-17
    Average 94 stars, based on 37 article reviews
    recombinant human epiregulin protein repr - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Potential involvement of Twist2 and Erk in the regulation of osteoblastogenesis by HB-EGF-EGFR signaling."

    Article Title: Potential involvement of Twist2 and Erk in the regulation of osteoblastogenesis by HB-EGF-EGFR signaling.

    Journal: Cell structure and function

    doi: 10.1247/csf.10001

    Fig. 1. EGFR signaling suppresses osteoblast differentiation through Ras. A) Expression of EGF family members mRNA in osteoblasts. MC3T3-E1 cells were induced to differentiate into osteoblasts, and the mRNA expression of EGF family members was monitored by RT-PCR at the indicated time points. GAPDH was used as an internal control. Hb-egf, heparin-biding EGF-like growth factor; Tgfα, transforming growth factor-α; Areg, amphiregulin; Epr, epiregulin; Btc, betacellulin. P, positive control. mRNA from mouse heart, liver, and lung was used as a positive control. B) Effect of rHB-EGF on osteoblastogenesis. MC3T3-E1 cells were seeded on 24-well plates, and osteoblast differentiation was induced. Alkaline phosphatase (ALP) activity and mineralizing activity were measured by ALP staining (upper panel) and Alizarin red staining (lower panel), respectively, at the indicated time points. C) Expression of osteoblastic marker genes in MC3T3-E1 cells. Cells were cultured in osteogenic media in the presence of rHB-EGF, and the mRNA expression of the indicated genes was monitored by RT-PCR. ColI, Collagen type I; Alp, alkaline phosphatase; Opn, osteopontin; Ocn, osteocalcin. GAPDH was used as an internal control. D) Activation of ERK in osteoblast differentiation. The phosphorylated form of ERK was visualized by Western blotting. MC3T3-E1 cells were stimulated with rHB-EGF for 5 min in the presence of the indicated inhibitors. A.A., ascorbic acid; HB, rHB-EGF; αHB, anti-human HB-EGF neutralizing antibody; PD, PD98059, MEK inhibitor; AG, AG1478, EGFR inhibitor. E) MC3T3-E1 cells were cultured in osteogenic media stimulated with rHB-EGF and indicated inhibitors as shown in D) for 3 days and ALP staining was carried out. F) Involvement of Ras in osteoblast differentiation. A dominant-negative form of Ras was expressed in MC3T3-E1 cells, and ALP staining was performed three days after osteogenic stimulation in the presence or absence of rHB-EGF. Mock, empty vector.
    Figure Legend Snippet: Fig. 1. EGFR signaling suppresses osteoblast differentiation through Ras. A) Expression of EGF family members mRNA in osteoblasts. MC3T3-E1 cells were induced to differentiate into osteoblasts, and the mRNA expression of EGF family members was monitored by RT-PCR at the indicated time points. GAPDH was used as an internal control. Hb-egf, heparin-biding EGF-like growth factor; Tgfα, transforming growth factor-α; Areg, amphiregulin; Epr, epiregulin; Btc, betacellulin. P, positive control. mRNA from mouse heart, liver, and lung was used as a positive control. B) Effect of rHB-EGF on osteoblastogenesis. MC3T3-E1 cells were seeded on 24-well plates, and osteoblast differentiation was induced. Alkaline phosphatase (ALP) activity and mineralizing activity were measured by ALP staining (upper panel) and Alizarin red staining (lower panel), respectively, at the indicated time points. C) Expression of osteoblastic marker genes in MC3T3-E1 cells. Cells were cultured in osteogenic media in the presence of rHB-EGF, and the mRNA expression of the indicated genes was monitored by RT-PCR. ColI, Collagen type I; Alp, alkaline phosphatase; Opn, osteopontin; Ocn, osteocalcin. GAPDH was used as an internal control. D) Activation of ERK in osteoblast differentiation. The phosphorylated form of ERK was visualized by Western blotting. MC3T3-E1 cells were stimulated with rHB-EGF for 5 min in the presence of the indicated inhibitors. A.A., ascorbic acid; HB, rHB-EGF; αHB, anti-human HB-EGF neutralizing antibody; PD, PD98059, MEK inhibitor; AG, AG1478, EGFR inhibitor. E) MC3T3-E1 cells were cultured in osteogenic media stimulated with rHB-EGF and indicated inhibitors as shown in D) for 3 days and ALP staining was carried out. F) Involvement of Ras in osteoblast differentiation. A dominant-negative form of Ras was expressed in MC3T3-E1 cells, and ALP staining was performed three days after osteogenic stimulation in the presence or absence of rHB-EGF. Mock, empty vector.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Positive Control, Activity Assay, Staining, Marker, Cell Culture, Activation Assay, Western Blot, Dominant Negative Mutation, Plasmid Preparation

    Related Articles

    Recombinant:

    Article Title: Potential involvement of Twist2 and Erk in the regulation of osteoblastogenesis by HB-EGF-EGFR signaling.
    Article Snippet: Ascorbic acid and glycerol 2-phosphate were obtained from Sigma. .. Recombinant human HB-EGF protein (rHB-EGF), recombinant human epiregulin protein (rEpr) and anti-human HB-EGF antibody were purchased from R&D Systems. .. AG1478, PD98059, and LY294002 were purchased from Calbiochem.



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    recombinant human epiregulin protein repr  (R&D Systems)
    94
    R&D Systems recombinant human epiregulin protein repr
    Fig. 1. EGFR signaling suppresses osteoblast differentiation through Ras. A) Expression of EGF family members mRNA in osteoblasts. MC3T3-E1 cells were induced to differentiate into osteoblasts, and the mRNA expression of EGF family members was monitored by RT-PCR at the indicated time points. GAPDH was used as an internal control. Hb-egf, heparin-biding EGF-like growth factor; Tgfα, transforming growth factor-α; Areg, amphiregulin; Epr, <t>epiregulin;</t> Btc, betacellulin. P, positive control. mRNA from mouse heart, liver, and lung was used as a positive control. B) Effect of rHB-EGF on osteoblastogenesis. MC3T3-E1 cells were seeded on 24-well plates, and osteoblast differentiation was induced. Alkaline phosphatase (ALP) activity and mineralizing activity were measured by ALP staining (upper panel) and Alizarin red staining (lower panel), respectively, at the indicated time points. C) Expression of osteoblastic marker genes in MC3T3-E1 cells. Cells were cultured in osteogenic media in the presence of rHB-EGF, and the mRNA expression of the indicated genes was monitored by RT-PCR. ColI, Collagen type I; Alp, alkaline phosphatase; Opn, osteopontin; Ocn, osteocalcin. GAPDH was used as an internal control. D) Activation of ERK in osteoblast differentiation. The phosphorylated form of ERK was visualized by Western blotting. MC3T3-E1 cells were stimulated with rHB-EGF for 5 min in the presence of the indicated inhibitors. A.A., ascorbic acid; HB, rHB-EGF; αHB, anti-human HB-EGF neutralizing antibody; PD, PD98059, MEK inhibitor; AG, AG1478, EGFR inhibitor. E) MC3T3-E1 cells were cultured in osteogenic media stimulated with rHB-EGF and indicated inhibitors as shown in D) for 3 days and ALP staining was carried out. F) Involvement of Ras in osteoblast differentiation. A dominant-negative form of Ras was expressed in MC3T3-E1 cells, and ALP staining was performed three days after osteogenic stimulation in the presence or absence of rHB-EGF. Mock, empty vector.
    Recombinant Human Epiregulin Protein Repr, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+epiregulin+protein+repr/Recombinant+Human+Epiregulin+Protein/pm20448405-37-5-17
    Average 94 stars, based on 1 article reviews
    recombinant human epiregulin protein repr - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

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    Fig. 1. EGFR signaling suppresses osteoblast differentiation through Ras. A) Expression of EGF family members mRNA in osteoblasts. MC3T3-E1 cells were induced to differentiate into osteoblasts, and the mRNA expression of EGF family members was monitored by RT-PCR at the indicated time points. GAPDH was used as an internal control. Hb-egf, heparin-biding EGF-like growth factor; Tgfα, transforming growth factor-α; Areg, amphiregulin; Epr, epiregulin; Btc, betacellulin. P, positive control. mRNA from mouse heart, liver, and lung was used as a positive control. B) Effect of rHB-EGF on osteoblastogenesis. MC3T3-E1 cells were seeded on 24-well plates, and osteoblast differentiation was induced. Alkaline phosphatase (ALP) activity and mineralizing activity were measured by ALP staining (upper panel) and Alizarin red staining (lower panel), respectively, at the indicated time points. C) Expression of osteoblastic marker genes in MC3T3-E1 cells. Cells were cultured in osteogenic media in the presence of rHB-EGF, and the mRNA expression of the indicated genes was monitored by RT-PCR. ColI, Collagen type I; Alp, alkaline phosphatase; Opn, osteopontin; Ocn, osteocalcin. GAPDH was used as an internal control. D) Activation of ERK in osteoblast differentiation. The phosphorylated form of ERK was visualized by Western blotting. MC3T3-E1 cells were stimulated with rHB-EGF for 5 min in the presence of the indicated inhibitors. A.A., ascorbic acid; HB, rHB-EGF; αHB, anti-human HB-EGF neutralizing antibody; PD, PD98059, MEK inhibitor; AG, AG1478, EGFR inhibitor. E) MC3T3-E1 cells were cultured in osteogenic media stimulated with rHB-EGF and indicated inhibitors as shown in D) for 3 days and ALP staining was carried out. F) Involvement of Ras in osteoblast differentiation. A dominant-negative form of Ras was expressed in MC3T3-E1 cells, and ALP staining was performed three days after osteogenic stimulation in the presence or absence of rHB-EGF. Mock, empty vector.

    Journal: Cell structure and function

    Article Title: Potential involvement of Twist2 and Erk in the regulation of osteoblastogenesis by HB-EGF-EGFR signaling.

    doi: 10.1247/csf.10001

    Figure Lengend Snippet: Fig. 1. EGFR signaling suppresses osteoblast differentiation through Ras. A) Expression of EGF family members mRNA in osteoblasts. MC3T3-E1 cells were induced to differentiate into osteoblasts, and the mRNA expression of EGF family members was monitored by RT-PCR at the indicated time points. GAPDH was used as an internal control. Hb-egf, heparin-biding EGF-like growth factor; Tgfα, transforming growth factor-α; Areg, amphiregulin; Epr, epiregulin; Btc, betacellulin. P, positive control. mRNA from mouse heart, liver, and lung was used as a positive control. B) Effect of rHB-EGF on osteoblastogenesis. MC3T3-E1 cells were seeded on 24-well plates, and osteoblast differentiation was induced. Alkaline phosphatase (ALP) activity and mineralizing activity were measured by ALP staining (upper panel) and Alizarin red staining (lower panel), respectively, at the indicated time points. C) Expression of osteoblastic marker genes in MC3T3-E1 cells. Cells were cultured in osteogenic media in the presence of rHB-EGF, and the mRNA expression of the indicated genes was monitored by RT-PCR. ColI, Collagen type I; Alp, alkaline phosphatase; Opn, osteopontin; Ocn, osteocalcin. GAPDH was used as an internal control. D) Activation of ERK in osteoblast differentiation. The phosphorylated form of ERK was visualized by Western blotting. MC3T3-E1 cells were stimulated with rHB-EGF for 5 min in the presence of the indicated inhibitors. A.A., ascorbic acid; HB, rHB-EGF; αHB, anti-human HB-EGF neutralizing antibody; PD, PD98059, MEK inhibitor; AG, AG1478, EGFR inhibitor. E) MC3T3-E1 cells were cultured in osteogenic media stimulated with rHB-EGF and indicated inhibitors as shown in D) for 3 days and ALP staining was carried out. F) Involvement of Ras in osteoblast differentiation. A dominant-negative form of Ras was expressed in MC3T3-E1 cells, and ALP staining was performed three days after osteogenic stimulation in the presence or absence of rHB-EGF. Mock, empty vector.

    Article Snippet: Recombinant human HB-EGF protein (rHB-EGF), recombinant human epiregulin protein (rEpr) and anti-human HB-EGF antibody were purchased from R&D Systems.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Positive Control, Activity Assay, Staining, Marker, Cell Culture, Activation Assay, Western Blot, Dominant Negative Mutation, Plasmid Preparation